Conditional poliovirus mutants made by random deletion mutagenesis of infectious cDNA
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چکیده
منابع مشابه
Conditional poliovirus mutants made by random deletion mutagenesis of infectious cDNA.
Small deletions were introduced into DNA plasmids bearing cDNA copies of Mahoney type 1 poliovirus RNA. The procedure used was similar to that of P. Hearing and T. Shenk (J. Mol. Biol. 167:809-822, 1983), with modifications designed to introduce only one lesion randomly into each DNA molecule. Methods to map small deletions in either large DNA or RNA molecules were employed. Two poliovirus muta...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1990
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.64.1.185-194.1990